Fertilisation, Embryo Culture and Transfer

Fertilisation:

Depending on the semen quality, fertilisation is performed either by IVF or ICSI in order to provide as many fertilised oocytes as possible.

How to choose fertilisation media:

For optimal fertilisation results it is recommended to use Universal IVF as a dedicated fertilisation medium, since especially the high glucose content in Universal IVF aids sperm capacitation. However, all MediCult culture media contain glucose in a concentration sufficient for fertilisation and can therefore be used as fertilisation media. Depending on the chosen culture system fertilisation may therefore be performed in Universal IVF Medium, EmbryoAssist^TM, BlastAssist^® Medium 1 or ISM1^TM.

Embryo culture:

Oocytes and embryos are cultured in a medium providing physical and chemical conditions imitating the in vivo environment. As the embryo develops, its dependency on the medium environment changes; therefore a sequential media system is recommended.

How to choose culture media:

MediCult offers different culture systems:

• EmbryoAssist^TM for fertilisation and optimal culture of cleavage stage embryos until 4-8 cell stage.
• ISM1^TM for short term (< 4 hrs) fertilisation and optimal culture of cleavage stage embryos until 2-8 cell stage.
• ISM2^TM for blastocyst culture after embryo selection in ISM1^TM.
• BlastAssist^® System. The BlastAssist^® System is the optimal sequential media system for dedicated blastocyst culture.

Products in this group:

• Universal IVF Medium
• EmbryoAssist™ 
• BlastAssist^®System
• ISM1™, ISM2™, UTM™